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PML induces compaction, TRF2 depletion and DNA damage signaling at telomeres and promotes their alternative lengthening

Overview of attention for article published in Journal of Cell Science, May 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (84th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

Mentioned by

news
1 news outlet
twitter
2 tweeters

Readers on

mendeley
51 Mendeley
Title
PML induces compaction, TRF2 depletion and DNA damage signaling at telomeres and promotes their alternative lengthening
Published in
Journal of Cell Science, May 2015
DOI 10.1242/jcs.148296
Pubmed ID
Authors

Osterwald, Sarah, Deeg, Katharina I, Chung, Inn, Parisotto, Daniel, Wörz, Stefan, Rohr, Karl, Erfle, Holger, Rippe, Karsten

Abstract

The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated PML nuclear bodies (APBs) at telomeres. We dissected the role of APBs in a human ALT cell line by RNA interference with an automated 3D fluorescence microscopy platform in conjunction with advanced 3D image analysis. We identified 29 proteins that affected APB formation that were involved in telomere and chromatin organization, protein sumoylation and DNA repair. By integrating and extending these findings we found that APB formation induced clustering of telomere repeats, telomere compaction and concomitant depletion of the shelterin protein TRF2. These APB-dependent changes correlated with the induction of a DNA damage response at colocalizing telomeres via a strong enrichment of the phosphorylated form of the ataxia telangiectasia mutated (ATM) kinase. Accordingly, we propose that APBs promote telomere maintenance by inducing a DNA damage response in ALT-positive tumor cells via changing the telomeric chromatin state to trigger ATM phosphorylation.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 2%
Brazil 1 2%
Unknown 49 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 27%
Researcher 12 24%
Student > Master 11 22%
Student > Bachelor 8 16%
Student > Postgraduate 3 6%
Other 3 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 57%
Biochemistry, Genetics and Molecular Biology 14 27%
Medicine and Dentistry 3 6%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Unspecified 1 2%
Other 3 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2015.
All research outputs
#538,310
of 5,221,989 outputs
Outputs from Journal of Cell Science
#104
of 2,742 outputs
Outputs of similar age
#27,053
of 172,755 outputs
Outputs of similar age from Journal of Cell Science
#6
of 76 outputs
Altmetric has tracked 5,221,989 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,742 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 172,755 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 84% of its contemporaries.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.