| Title |
Real-time observation of light-controlled transcription in living cells
|
|---|---|
| Published in |
Journal of Cell Science, January 2017
|
| DOI | 10.1242/jcs.205534 |
| Pubmed ID | |
| Authors |
Anne Rademacher, Fabian Erdel, Jorge Trojanowski, Sabrina Schumacher, Karsten Rippe |
| Abstract |
Gene expression is tightly regulated in space and time. To dissect this process with high temporal resolution, we introduce an optogenetic tool termed BLInCR (Blue Light-Induced Chromatin Recruitment) that combines rapid and reversible light-dependent recruitment of effector proteins with a real-time readout for transcription. We used BLInCR to control the activity of a reporter gene cluster in the human osteosarcoma cell line U2OS by reversibly recruiting the viral transactivator VP16. RNA production was detectable ∼2 minutes after VP16 recruitment and readily decreased when VP16 dissociated from the cluster in the absence of light. Quantitative assessment of the activation process revealed biphasic activation kinetics with a pronounced early phase in cells treated with the histone deacetylase inhibitor SAHA. Comparison with kinetic models for transcription activation suggests that the gene cluster undergoes a maturation process when activated. We anticipate that BLInCR will facilitate the study of transcription dynamics in living cells. |
X Demographics
The data shown below were collected from the profiles of 34 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 12 | 35% |
| Germany | 5 | 15% |
| Italy | 2 | 6% |
| France | 2 | 6% |
| Canada | 2 | 6% |
| United Kingdom | 2 | 6% |
| Norway | 1 | 3% |
| Switzerland | 1 | 3% |
| Australia | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 6 | 18% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 17 | 50% |
| Scientists | 16 | 47% |
| Practitioners (doctors, other healthcare professionals) | 1 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 108 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Unknown | 107 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 25 | 23% |
| Student > Bachelor | 18 | 17% |
| Researcher | 17 | 16% |
| Student > Master | 12 | 11% |
| Student > Postgraduate | 5 | 5% |
| Other | 11 | 10% |
| Unknown | 20 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 48 | 44% |
| Agricultural and Biological Sciences | 17 | 16% |
| Engineering | 5 | 5% |
| Medicine and Dentistry | 5 | 5% |
| Chemistry | 4 | 4% |
| Other | 7 | 6% |
| Unknown | 22 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 19. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2018.
All research outputs
#1,836,982
of 24,565,648 outputs
Outputs from Journal of Cell Science
#262
of 9,124 outputs
Outputs of similar age
#37,965
of 430,151 outputs
Outputs of similar age from Journal of Cell Science
#15
of 315 outputs
Altmetric has tracked 24,565,648 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,124 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 430,151 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 315 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.