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Real-time observation of light-controlled transcription in living cells

Overview of attention for article published in Journal of Cell Science, January 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

Mentioned by

twitter
36 tweeters

Citations

dimensions_citation
17 Dimensions

Readers on

mendeley
101 Mendeley
Title
Real-time observation of light-controlled transcription in living cells
Published in
Journal of Cell Science, January 2017
DOI 10.1242/jcs.205534
Pubmed ID
Authors

Anne Rademacher, Fabian Erdel, Jorge Trojanowski, Sabrina Schumacher, Karsten Rippe

Abstract

Gene expression is tightly regulated in space and time. To dissect this process with high temporal resolution, we introduce an optogenetic tool termed BLInCR (Blue Light-Induced Chromatin Recruitment) that combines rapid and reversible light-dependent recruitment of effector proteins with a real-time readout for transcription. We used BLInCR to control the activity of a reporter gene cluster in the human osteosarcoma cell line U2OS by reversibly recruiting the viral transactivator VP16. RNA production was detectable ∼2 minutes after VP16 recruitment and readily decreased when VP16 dissociated from the cluster in the absence of light. Quantitative assessment of the activation process revealed biphasic activation kinetics with a pronounced early phase in cells treated with the histone deacetylase inhibitor SAHA. Comparison with kinetic models for transcription activation suggests that the gene cluster undergoes a maturation process when activated. We anticipate that BLInCR will facilitate the study of transcription dynamics in living cells.

Twitter Demographics

The data shown below were collected from the profiles of 36 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 101 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 <1%
Unknown 100 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 24 24%
Researcher 17 17%
Student > Bachelor 17 17%
Student > Master 11 11%
Professor 6 6%
Other 9 9%
Unknown 17 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 43 43%
Agricultural and Biological Sciences 18 18%
Engineering 5 5%
Medicine and Dentistry 5 5%
Chemistry 4 4%
Other 6 6%
Unknown 20 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 20. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 February 2018.
All research outputs
#1,428,079
of 21,105,374 outputs
Outputs from Journal of Cell Science
#210
of 8,645 outputs
Outputs of similar age
#36,642
of 340,650 outputs
Outputs of similar age from Journal of Cell Science
#3
of 90 outputs
Altmetric has tracked 21,105,374 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,645 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.8. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 340,650 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 90 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.