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Interactions between the discoidin domain receptor 1 and β1 integrin regulate attachment to collagen.

Overview of attention for article published in Biology Open, January 2013
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3 tweeters

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mendeley
28 Mendeley
citeulike
1 CiteULike
Title
Interactions between the discoidin domain receptor 1 and β1 integrin regulate attachment to collagen.
Published in
Biology Open, January 2013
DOI 10.1242/bio.20135090
Pubmed ID
Authors

Lisa A Staudinger, Stephen J Spano, Wilson Lee, Nuno Coelho, Dhaarmini Rajshankar, Michelle P Bendeck, Tara Moriarty, Christopher A McCulloch, Staudinger LA, Spano SJ, Lee W, Coelho N, Rajshankar D, Bendeck MP, Moriarty T, McCulloch CA, Lisa A. Staudinger, Stephen J. Spano, Michelle P. Bendeck, Christopher A. McCulloch

Abstract

Collagen degradation by phagocytosis is essential for physiological collagen turnover and connective tissue homeostasis. The rate limiting step of phagocytosis is the binding of specific adhesion receptors, which include the integrins and discoidin domain receptors (DDR), to fibrillar collagen. While previous data suggest that these two receptors interact, the functional nature of these interactions is not defined. In mouse and human fibroblasts we examined the effects of DDR1 knockdown and over-expression on β1 integrin subunit function. DDR1 expression levels were positively associated with enhanced contraction of floating and attached collagen gels, increased collagen binding and increased collagen remodeling. In DDR1 over-expressing cells compared with control cells, there were increased numbers, area and length of focal adhesions immunostained for talin, paxillin, vinculin and activated β1 integrin. After treatment with the integrin-cleaving protease jararhagin, in comparison to controls, DDR1 over-expressing cells exhibited increased β1 integrin cleavage at the cell membrane, indicating that DDR1 over-expression affected the access and susceptibility of cell-surface β1 integrin to the protease. DDR1 over-expression was associated with increased glycosylation of the β1 integrin subunit, which when blocked by deoxymannojirimycin, reduced collagen binding. Collectively these data indicate that DDR1 regulates β1 integrin interactions with fibrillar collagen, which positively impacts the binding step of collagen phagocytosis and collagen remodeling.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 4%
United States 1 4%
Canada 1 4%
Unknown 25 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 36%
Researcher 6 21%
Student > Doctoral Student 3 11%
Student > Bachelor 2 7%
Student > Master 2 7%
Other 5 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 43%
Biochemistry, Genetics and Molecular Biology 6 21%
Medicine and Dentistry 3 11%
Engineering 3 11%
Unspecified 2 7%
Other 2 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2016.
All research outputs
#3,986,049
of 7,695,823 outputs
Outputs from Biology Open
#305
of 584 outputs
Outputs of similar age
#79,405
of 173,123 outputs
Outputs of similar age from Biology Open
#9
of 17 outputs
Altmetric has tracked 7,695,823 research outputs across all sources so far. This one is in the 28th percentile – i.e., 28% of other outputs scored the same or lower than it.
So far Altmetric has tracked 584 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 173,123 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.