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De novo assembly of a PML nuclear subcompartment occurs through multiple pathways and induces telomere elongation

Overview of attention for article published in Journal of Cell Science, January 2011
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (74th percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

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2 tweeters
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1 Facebook page

Readers on

mendeley
46 Mendeley
Title
De novo assembly of a PML nuclear subcompartment occurs through multiple pathways and induces telomere elongation
Published in
Journal of Cell Science, January 2011
DOI 10.1242/jcs.084681
Pubmed ID
Abstract

Telomerase-negative tumor cells use an alternative lengthening of telomeres (ALT) pathway that involves DNA recombination and repair to maintain their proliferative potential. The cytological hallmark of this process is the accumulation of promyelocytic leukemia (PML) nuclear protein at telomeric DNA to form ALT-associated PML bodies (APBs). Here, the de novo formation of a telomeric PML nuclear subcompartment was investigated by recruiting APB protein components. We show that functionally distinct proteins were able to initiate the formation of bona fide APBs with high efficiency in a self-organizing and self-propagating manner. These included: (1) PML and Sp100 as the constituting components of PML nuclear bodies, (2) telomere repeat binding factors 1 and 2 (TRF1 and TRF2, respectively), (3) the DNA repair protein NBS1 and (4) the SUMO E3 ligase MMS21, as well as the isolated SUMO1 domain, through an interacting domain of another protein factor. By contrast, the repair factors Rad9, Rad17 and Rad51 were less efficient in APB nucleation but were recruited to preassembled APBs. The artificially created APBs induced telomeric extension through a DNA repair mechanism, as inferred from their colocalization with sites of non-replicative DNA synthesis and histone H2A.X phosphorylation, and an increase of the telomere repeat length. These activities were absent after recruitment of the APB factors to a pericentric locus and establish APBs as functional intermediates of the ALT pathway.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 4%
Australia 1 2%
United Kingdom 1 2%
Poland 1 2%
Unknown 41 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 28%
Researcher 12 26%
Student > Master 6 13%
Student > Doctoral Student 6 13%
Professor 3 7%
Other 6 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 34 74%
Biochemistry, Genetics and Molecular Biology 7 15%
Medicine and Dentistry 3 7%
Immunology and Microbiology 1 2%
Engineering 1 2%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2011.
All research outputs
#1,450,995
of 3,635,018 outputs
Outputs from Journal of Cell Science
#1,082
of 2,328 outputs
Outputs of similar age
#16,588
of 69,413 outputs
Outputs of similar age from Journal of Cell Science
#1
of 12 outputs
Altmetric has tracked 3,635,018 research outputs across all sources so far. This one has received more attention than most of these and is in the 58th percentile.
So far Altmetric has tracked 2,328 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 69,413 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.