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De novo assembly of a PML nuclear subcompartment occurs through multiple pathways and induces telomere elongation

Overview of attention for article published in Journal of Cell Science, November 2011
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  • Average Attention Score compared to outputs of the same age
  • Good Attention Score compared to outputs of the same age and source (73rd percentile)

Mentioned by

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2 tweeters
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1 Facebook page

Citations

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56 Dimensions

Readers on

mendeley
93 Mendeley
Title
De novo assembly of a PML nuclear subcompartment occurs through multiple pathways and induces telomere elongation
Published in
Journal of Cell Science, November 2011
DOI 10.1242/jcs.084681
Pubmed ID
Authors

I. Chung, H. Leonhardt, K. Rippe

Abstract

Telomerase-negative tumor cells use an alternative lengthening of telomeres (ALT) pathway that involves DNA recombination and repair to maintain their proliferative potential. The cytological hallmark of this process is the accumulation of promyelocytic leukemia (PML) nuclear protein at telomeric DNA to form ALT-associated PML bodies (APBs). Here, the de novo formation of a telomeric PML nuclear subcompartment was investigated by recruiting APB protein components. We show that functionally distinct proteins were able to initiate the formation of bona fide APBs with high efficiency in a self-organizing and self-propagating manner. These included: (1) PML and Sp100 as the constituting components of PML nuclear bodies, (2) telomere repeat binding factors 1 and 2 (TRF1 and TRF2, respectively), (3) the DNA repair protein NBS1 and (4) the SUMO E3 ligase MMS21, as well as the isolated SUMO1 domain, through an interacting domain of another protein factor. By contrast, the repair factors Rad9, Rad17 and Rad51 were less efficient in APB nucleation but were recruited to preassembled APBs. The artificially created APBs induced telomeric extension through a DNA repair mechanism, as inferred from their colocalization with sites of non-replicative DNA synthesis and histone H2A.X phosphorylation, and an increase of the telomere repeat length. These activities were absent after recruitment of the APB factors to a pericentric locus and establish APBs as functional intermediates of the ALT pathway.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 2%
Poland 1 1%
Portugal 1 1%
Australia 1 1%
Unknown 88 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 20 22%
Researcher 18 19%
Student > Doctoral Student 10 11%
Student > Master 10 11%
Student > Bachelor 8 9%
Other 12 13%
Unknown 15 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 36 39%
Biochemistry, Genetics and Molecular Biology 33 35%
Immunology and Microbiology 3 3%
Medicine and Dentistry 3 3%
Economics, Econometrics and Finance 1 1%
Other 3 3%
Unknown 14 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2011.
All research outputs
#6,615,692
of 12,448,216 outputs
Outputs from Journal of Cell Science
#2,821
of 4,773 outputs
Outputs of similar age
#51,837
of 106,735 outputs
Outputs of similar age from Journal of Cell Science
#6
of 23 outputs
Altmetric has tracked 12,448,216 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,773 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 106,735 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.